University of Minnesota
Vision Research at UMN
http://vision.umn.edu

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Core Facilities

The services and facilities of three vision core grant modules are available, free of charge, to all core grant investigators. General questions concerning the core should be directed to its principal investigator, Dr. Eric Newman.

Digital Imaging Module

The Digital Imaging module offers two major services to Core Grant investigators: (1) a confocal facility utilizing an Olympus FV1000 confocal microscope and a Prairie Technologies multi-photon microscope, and (2) an image analysis facility.  The module is managed by Dr. Terry Wu, who maintains the confocal and multi-photon microscopes, provides training to Core Grant investigators and staff on use of the microscopes, maintains the digital image processing and analysis equipment, trains Core Grant investigators and staff on the use of image processing and analysis equipment, and performs complex imaging processing tasks for Core Grant investigators.

Information Technology Module

The Information Technology (IT) module has two major functions: (1) it offers the services of our IT manager, Mr. Greg Smith, for custom software design and assistance with computer- and network-related issues, and (2) it provides the infrastructure and administration of the Vision Core Grant network.  The network infrastructure includes dedicated computer servers for networking and archiving of critical experimental data.

Histology Module

The Histology module provides a fully equipped histology laboratory and the services of the module histologist to Core Grant investigators and staff.  Services offered by our skilled histologist, Ms. Heidi Rohrich, include (1) sectioning of paraffin embedded and frozen tissue, (2) hematoxylin and eosin staining of sections and other histological stains as required (e.g. Oil Red O, cresyl violet), (3) immunohistochemistry using both DAB and fluorescence based procedures, (4) in situ hybridization, (5) nuclease protection assays, (6) RNA extraction, (7) protein assays, (8) image acquisition and analysis using the Bioquant Nova Prime software, and (9) preparation of publication quality photomicrographs using Photoshop.